199 – Mass Spectrometers
Guest: Alexander Makarov Host: Markus Voelter Shownoter: Bastian Hundt
Mass spectrometers are devices for measuring the mass-to-charge ratio of molecules and ions. They use many different measurement principles and are used in various areas of science. Our guest Alexander Makarov works as a Director Global Research for Thermo Fisher‘s Life Sciences Division and has invented the Orbitrap principle used widely in modern mass spectrometers. We talk about mass spectrometry in general, the different measurement principles, engineering challenges, the invention of the Orbitrap, use cases for mass spectrometers and the different machines sold by Thermo Fisher.
Introduction of Alexander Makarov00:01:18
Introduction to Mass-Spectrometers00:06:59
Mass Spectrometers | Mass | Electromagnetic field | Ionization | Mass-To-Charge ratio | Matrix-assisted laser desorption/ionization | Electrospray Ionization | Deconvolution | Carbon-13 | Isotope analysis | Ernst Rutherford | Isotope-ratio mass spectrometry
Description of a Mass Spectrometer00:27:48
Signal Processing < | Electro ionization | Chemical ionization | Gas chromatography | Liquid chromatography mass spectrometry | Quadrupole mass analyzer | Targeted mass spectroscopy | Time-of-flight mass spectroscopy | Ion optics / Electrostatic lens | Ion trap mass analyzers | Fourier transform ion cyclotron resonance
Orbitrap | Spark ionization | Ion trap | Chaos theory | Nonlinear system | DNA
Requirements for commercial Mass Spectrometers01:10:48
Linear Response | Accuracy and prescision | Resolution in a mass spectrometer | Dynamic range | Quantification | Quantitative analysis
Challenges of modern Mass Spectrometers01:32:38
Tour of the Factory / R&D01:43:33
Really cool stuff, and the inventor talking himself.
This could not have been better.
Thank you very much.
Great episode, I went the next day and pissed off my chemist colleagues by demanding to see MS data and assess the purity of their stuff :D